Gene analysis of reassortant influenza virus by RT-PCR followed by restriction enzyme digestion
Identifieur interne : 001C27 ( Main/Exploration ); précédent : 001C26; suivant : 001C28Gene analysis of reassortant influenza virus by RT-PCR followed by restriction enzyme digestion
Auteurs : Shinichi Sakamoto [Japon] ; Yoichiro Kino [Japon] ; Testuya Oka [Japon] ; M. Louise Herlocher [États-Unis] ; Hunien F. Maassab [États-Unis]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 1996.
Descripteurs français
- KwdFr :
- ADN complémentaire, ADN viral (analyse), ADN viral (métabolisme), ARN viral (analyse), Deoxyribonuclease BamHI (métabolisme), Desoxyribonuclease HpaII (métabolisme), Données de séquences moléculaires, Humains, Réaction de polymérisation en chaîne (), Séquence nucléotidique, Type II site-specific deoxyribonuclease (métabolisme), Virus de la grippe A (génétique), Virus influenza B (génétique), Virus recombinants (génétique).
- MESH :
- analyse : ADN viral, ARN viral.
- génétique : Virus de la grippe A, Virus influenza B, Virus recombinants.
- métabolisme : ADN viral, Deoxyribonuclease BamHI, Desoxyribonuclease HpaII, Type II site-specific deoxyribonuclease.
- Pascal (Inist)
- ADN complémentaire, Amplification génique, Données de séquences moléculaires, Humains, Influenzavirus, Méthode analyse, Organisation génome, RNA, Réaction chaîne polymérase, Réaction de polymérisation en chaîne, Réassortiment génétique, Souche atténuée, Souche vaccinale, Séquence nucléotidique, Virus recombinant.
English descriptors
- KwdEn :
- Analysis method, Attenuated strain, Base Sequence, DNA, Complementary, DNA, Viral (analysis), DNA, Viral (metabolism), Deoxyribonuclease BamHI (metabolism), Deoxyribonuclease HpaII (metabolism), Deoxyribonucleases, Type II Site-Specific (metabolism), Gene amplification, Genetic reassortment, Genome organization, Humans, Influenza A virus (genetics), Influenza B virus (genetics), Influenzavirus, Molecular Sequence Data, Polymerase Chain Reaction (methods), Polymerase chain reaction, RNA, RNA, Viral (analysis), Reassortant Viruses (genetics), Recombinant virus, Vaccine strain.
- MESH :
- chemical , analysis : DNA, Viral, RNA, Viral.
- chemical , metabolism : DNA, Viral, Deoxyribonuclease BamHI, Deoxyribonuclease HpaII, Deoxyribonucleases, Type II Site-Specific.
- chemical : DNA, Complementary.
- genetics : Influenza A virus, Influenza B virus, Reassortant Viruses.
- methods : Polymerase Chain Reaction.
- Base Sequence, Humans, Molecular Sequence Data.
- Teeft :
- Agarose, Amplification, Amplification products, Amplification system, Cdna, Cdna synthesis, Digestion, Digestion patterns, Genome, Genome analysis, Influenza, Influenza virus, Influenza virus genome, Influenza viruses, Other viruses, Primer, Reassortant, Reassortant viruses, Restriction enzymes, Sakamoto, Syuzou, Takara, Takara syuzou, Vaccine, Vaccine production, Virological, Virological methods, Virus, Wild virus, Wild viruses.
Abstract
Abstract: An amplification system for nearly full length cDNA coding the eight influenza virus segments of A type (H1N1, H2N2, H3N2) and B type influenza viruses is described. Each of the segments of PB1, PB2, PA, NP, M, and NS can be amplified using one 5′ primer and one 3′ primer for A-type influenza viruses. The RT-PCR amplification system was applied to define the gene composition of three subtype cold-recombinant, live attenuated influenza viruses. Each segment of the attenuated influenza virus could be identified as deriving from segments of the Ca donor or wild virus by comparing the representative restriction enzyme digestion patterns of the three PCR products obtained from the Ca donor, the cold-live attenuated influenza viruses and the wild virus. This RT-PCR method, using RT-PCR followed by digestion of PCR products with restriction enzymes, was very beneficial for analyzing the genome of reassortant influenza viruses.
Url:
DOI: 10.1016/0166-0934(95)01909-X
Affiliations:
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Le document en format XML
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Louise" last="Herlocher">M. Louise Herlocher</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Michigan School of Public Health, Ann Arbor, Michigan 48109</wicri:regionArea><wicri:noRegion>Michigan 48109</wicri:noRegion></affiliation></author><author><name sortKey="Maassab, Hunien F" sort="Maassab, Hunien F" uniqKey="Maassab H" first="Hunien F." last="Maassab">Hunien F. Maassab</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Michigan School of Public Health, Ann Arbor, Michigan 48109</wicri:regionArea><wicri:noRegion>Michigan 48109</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="ISSN">0166-0934</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">56</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="161">161</biblScope><biblScope unit="page" to="171">171</biblScope></imprint><idno type="ISSN">0166-0934</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analysis method</term><term>Attenuated strain</term><term>Base Sequence</term><term>DNA, Complementary</term><term>DNA, Viral (analysis)</term><term>DNA, Viral (metabolism)</term><term>Deoxyribonuclease BamHI (metabolism)</term><term>Deoxyribonuclease HpaII (metabolism)</term><term>Deoxyribonucleases, Type II Site-Specific (metabolism)</term><term>Gene amplification</term><term>Genetic reassortment</term><term>Genome organization</term><term>Humans</term><term>Influenza A virus (genetics)</term><term>Influenza B virus (genetics)</term><term>Influenzavirus</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction (methods)</term><term>Polymerase chain reaction</term><term>RNA</term><term>RNA, Viral (analysis)</term><term>Reassortant Viruses (genetics)</term><term>Recombinant virus</term><term>Vaccine strain</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire</term><term>ADN viral (analyse)</term><term>ADN viral (métabolisme)</term><term>ARN viral (analyse)</term><term>Deoxyribonuclease BamHI (métabolisme)</term><term>Desoxyribonuclease HpaII (métabolisme)</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Réaction de polymérisation en chaîne ()</term><term>Séquence nucléotidique</term><term>Type II site-specific deoxyribonuclease (métabolisme)</term><term>Virus de la grippe A (génétique)</term><term>Virus influenza B (génétique)</term><term>Virus recombinants (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Viral</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Viral</term><term>Deoxyribonuclease BamHI</term><term>Deoxyribonuclease HpaII</term><term>Deoxyribonucleases, Type II Site-Specific</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN viral</term><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term><term>Influenza B virus</term><term>Reassortant Viruses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de la grippe A</term><term>Virus influenza B</term><term>Virus recombinants</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN viral</term><term>Deoxyribonuclease BamHI</term><term>Desoxyribonuclease HpaII</term><term>Type II site-specific deoxyribonuclease</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Humans</term><term>Molecular Sequence Data</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>ADN complémentaire</term><term>Amplification génique</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Influenzavirus</term><term>Méthode analyse</term><term>Organisation génome</term><term>RNA</term><term>Réaction chaîne polymérase</term><term>Réaction de polymérisation en chaîne</term><term>Réassortiment génétique</term><term>Souche atténuée</term><term>Souche vaccinale</term><term>Séquence nucléotidique</term><term>Virus recombinant</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Agarose</term><term>Amplification</term><term>Amplification products</term><term>Amplification system</term><term>Cdna</term><term>Cdna synthesis</term><term>Digestion</term><term>Digestion patterns</term><term>Genome</term><term>Genome analysis</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus genome</term><term>Influenza viruses</term><term>Other viruses</term><term>Primer</term><term>Reassortant</term><term>Reassortant viruses</term><term>Restriction enzymes</term><term>Sakamoto</term><term>Syuzou</term><term>Takara</term><term>Takara syuzou</term><term>Vaccine</term><term>Vaccine production</term><term>Virological</term><term>Virological methods</term><term>Virus</term><term>Wild virus</term><term>Wild viruses</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: An amplification system for nearly full length cDNA coding the eight influenza virus segments of A type (H1N1, H2N2, H3N2) and B type influenza viruses is described. Each of the segments of PB1, PB2, PA, NP, M, and NS can be amplified using one 5′ primer and one 3′ primer for A-type influenza viruses. The RT-PCR amplification system was applied to define the gene composition of three subtype cold-recombinant, live attenuated influenza viruses. Each segment of the attenuated influenza virus could be identified as deriving from segments of the Ca donor or wild virus by comparing the representative restriction enzyme digestion patterns of the three PCR products obtained from the Ca donor, the cold-live attenuated influenza viruses and the wild virus. This RT-PCR method, using RT-PCR followed by digestion of PCR products with restriction enzymes, was very beneficial for analyzing the genome of reassortant influenza viruses.</div></front></TEI><affiliations><list><country><li>Japon</li><li>États-Unis</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Sakamoto, Shinichi" sort="Sakamoto, Shinichi" uniqKey="Sakamoto S" first="Shinichi" last="Sakamoto">Shinichi Sakamoto</name></noRegion><name sortKey="Kino, Yoichiro" sort="Kino, Yoichiro" uniqKey="Kino Y" first="Yoichiro" last="Kino">Yoichiro Kino</name><name sortKey="Oka, Testuya" sort="Oka, Testuya" uniqKey="Oka T" first="Testuya" last="Oka">Testuya Oka</name></country><country name="États-Unis"><noRegion><name sortKey="Herlocher, M Louise" sort="Herlocher, M Louise" uniqKey="Herlocher M" first="M. Louise" last="Herlocher">M. Louise Herlocher</name></noRegion><name sortKey="Maassab, Hunien F" sort="Maassab, Hunien F" uniqKey="Maassab H" first="Hunien F." last="Maassab">Hunien F. Maassab</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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